Limbach Group Biological Mass Spectrometry

A significant component of our collaborative research revolves around the characterization of enzymes or enzyme complexes that are involved in the posttranscriptional modification of RNA. Selected publications below highlight the diversity of these collaborative projects.

Selected Publications

• van Tran, N.; Muller, L.; Ross, R.L.; Lestini, R.; Létoquart, J.; Ulryck, N.; Limbach, P.A.; de Crécy-Lagard, V.; Cianférani, S.; Graille, M.; “Evolutionary insights into Trm112-methyltransferase holoenzymes involved in translation between archaea and eukaryotes” Nucleic Acids Research (2018) 46 8483-8499. PMID: 30010922
• Baldridge, K.C.; Maranhao, A.C.; Jora, M.; Quick, M.M.; Brodbelt, J.S.; Limbach, P.A.; Ellington, A.D.; Contreras, L.M.; “Directed evolution of heterologous tRNAs leads to independence from post-transcriptional modifications” ACS Synthetic Biology (2018) 7 1315-1327. PMID: 29694026
• Wang, W-P.; Ho, P-Y.; Chen, Q. Addepalli, B.; Limbach, P.A.; Li, M.; Wu, W.; Jilek, J.L.; Qiu, J.; Zhang, H.; Li, T.; Wun, T.; Lam, K.S.; Yu, A-Y.; “Bioengineering novel chimeric microRNA-34a for prodrug cancer therapy: High-yield expression and purification, and structural and functional characterization” The Journal of Pharmacology and Experimental Therapeutics (2015) 354 131-41. PMID: 26022002     
  
• Rodriguez-Hernandez, A.; Spears, J.; Gaston, K.W.; Limbach, P.A.; Gamper, H.; Hou, Y.-M.; Agris, P.; Perona, J.J.; “Structural and mechanistic basis for enhanced translational efficiency by 2-thiouridine at the tRNA anticodon wobble position” Journal of Molecular Biology 425 (2013) 3888-3906. PMID: 23727144    
• Guy, M.P.; Podyma, B.M.; Preston, M.A.; Shaheen, H.H.; Krivos, K.L.; Limbach, P.A.; Hopper, A.K.; Phizicky, E.M.; “Distinct Trm7 complexes that direct anticodon loop modifications are required for efficient tRNAPhe function and healthy growth in yeast”, RNA 18 (2012) 1921-1933.  PMID: 22912484 
• Aldinger, C.A.; Leisinger, A-K; Gaston, K.W.; Limbach, P.A.; Igloi, G.L.; “The absence of A-to-I editing in the anticodon of plant cytoplasmic tRNAArgACGdemands a relaxation of the wobble rules in plants”, RNA Biology 9 (2012) 1239-1246. PMID: 22922796    
• Phillips, G.; Swairjo, M.A.; Gaston, K.W.; Bailly, M.; Iwata-Reuyl, D.; Limbach, P.A.; de Crecy-Lagard, V.; “Diversity of Archaeosine Synthesis in Crenarchaeota”, ACS Chemical Biology, 7 (2012) 300-305. PMID: 22032275.    
• Mandal, D., Köhrer, C., Su, D., Russell, S.P., Krivos, K., Castleberry, C.M., Blum, P., Limbach, P.A., Söll, D., RajBhandary, U.L. “Agmatidine, a modified cytidine in the anticodon of archaeal tRNA^Ile, base pairs with Adenosine but not with Guanosine”, Proceedings of the National Academy of Sciences, USA 107 (2010) 2872-2877. PMID: 20133752.