Limbach Group Biological Mass Spectrometry

A Member of the Rieveschl Laboratories for Mass Spectrometry

in the College of Arts & Sciences' Department of Chemistry

RAMM Software Update (v2) now available


RoboOligo Software now available


Learn more about the National Academies Consensus study on Sequencing the Epitranscriptome


Selected Recent Publications

Robert L. Ross, Ningxi Yu, Ruoxia Zhao, Andrew Wood and Patrick A. Limbach. “Automated Identification of Modified Nucleosides during HRAM-LC-MS/MS using a Metabolomics ID Workflow with Neutral Loss Detection” Journal of the American Society for Mass Spectrometry (2023) doi:10.1021/jasms.3c00298.


Manasses Jora, Daniel Corcoran, Gwenn G. Parungao, Peter A. Lobue, Luiz F. L. Oliveira, George Stan, Balasubrahmanyam Addepalli, and Patrick A. Limbach “Higher-Energy Collisional Dissociation Mass Spectral Networks for the Rapid, Semi-automated Characterization of Known and Unknown Ribonucleoside Modifications” Analytical Chemistry (2022) 94(40):13958-13967. doi: 10.1021/acs.analchem.2c03172. PMID: 36174068.


"Chemical Amination/Imination of Carbonothiolated Nucleosides During RNA Hydrolysis” Jora M, Borland K, Abernathy S, Zhao R, Kelley M, Kellner S, Addepalli B, Limbach PA. Angew Chem Int Ed Engl. (2021) 60 3961-3966. DOI: 10.1002/anie.202010793. PMID: 33125801


“Oligonucleotide Analysis by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry in the Absence of Ion-Pair Reagents” P.A. Lobue, M. Jora, B. Addepalli, P.A. Limbach,  Journal of Chromatography A (2019) DOI: 10.1016/j.chroma.2019.02.016. PMID: 30772056.    


“Transfer RNA Modification Profiles and Codon Decoding Strategies in Methanocaldococcus jannaschii” N. Yu, M. Jora, B. Solivio, P. Thakur, C.G. Acevedo-Rocha, L. Randau, V. de Crécy-Lagard, B. Addepalli, P.A. Limbach,  Journal of Bacteriology (2019) 201 e00690-18. DOI: 10.1128/JB.00690-18 PMID: 30745370.


“RNAModMapper: RNA modification mapping software for analysis of mass spectrometry data”, N Yu, C Wetzel, X Cao, PA Limbach, Analytical Chemistry (2017) doi: 10.1021/acs.analchem.7b01780 PMID: 28942636


June 2 - 6, 2024

Future ASMS conference in Anaheim, CA


Upcoming Events

Limbach Group - Home

The primary interest of Prof. Pat Limbach and members of his research group is to develop new and improved mass spectrometric methods to characterize modified ribonucleic acids (RNAs) and ribonucleoprotein complexes (RNPs). As such, we have active research programs involved in nucleic acid analysis by mass spectrometry, structural biology and medical applications. Details can be accessed below.


Sequencing of Modified RNAs by Mass Spectrometry

Biological Studies of Modified RNA Nucleosides

Structural Characterization of RNA:Protein Complexes

Medical Research Impacts of Modified RNAs


Our research in these areas has been or is generously supported by the following funding agencies and companies.


If you have any questions, would like to discuss our research program, or are interested in supporting our research or your organization would benefit from the talents and expertise of our group, please contact Professor Limbach via phone (513-556-1871) or email (Pat.Limbach at uc.edu).

The following information about our research program at the University of Cincinnati is available on this site:


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