Limbach Group Biological Mass Spectrometry

A Member of the Rieveschl Laboratories for Mass Spectrometry

in the College of Arts & Sciences' Department of Chemistry

RAMM Software Update (v2) now available

RoboOligo Software now available

Learn more about the National Academies Consensus study on Sequencing the Epitranscriptome

Selected Recent Publications

Robert L. Ross, Ningxi Yu, Ruoxia Zhao, Andrew Wood and Patrick A. Limbach. “Automated Identification of Modified Nucleosides during HRAM-LC-MS/MS using a Metabolomics ID Workflow with Neutral Loss Detection” Journal of the American Society for Mass Spectrometry (2023) doi:10.1021/jasms.3c00298.

Manasses Jora, Daniel Corcoran, Gwenn G. Parungao, Peter A. Lobue, Luiz F. L. Oliveira, George Stan, Balasubrahmanyam Addepalli, and Patrick A. Limbach “Higher-Energy Collisional Dissociation Mass Spectral Networks for the Rapid, Semi-automated Characterization of Known and Unknown Ribonucleoside Modifications” Analytical Chemistry (2022) 94(40):13958-13967. doi: 10.1021/acs.analchem.2c03172. PMID: 36174068.

"Chemical Amination/Imination of Carbonothiolated Nucleosides During RNA Hydrolysis” Jora M, Borland K, Abernathy S, Zhao R, Kelley M, Kellner S, Addepalli B, Limbach PA. Angew Chem Int Ed Engl. (2021) 60 3961-3966. DOI: 10.1002/anie.202010793. PMID: 33125801

“Oligonucleotide Analysis by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry in the Absence of Ion-Pair Reagents” P.A. Lobue, M. Jora, B. Addepalli, P.A. Limbach,  Journal of Chromatography A (2019) DOI: 10.1016/j.chroma.2019.02.016. PMID: 30772056.    

“Transfer RNA Modification Profiles and Codon Decoding Strategies in Methanocaldococcus jannaschii” N. Yu, M. Jora, B. Solivio, P. Thakur, C.G. Acevedo-Rocha, L. Randau, V. de Crécy-Lagard, B. Addepalli, P.A. Limbach,  Journal of Bacteriology (2019) 201 e00690-18. DOI: 10.1128/JB.00690-18 PMID: 30745370.

“RNAModMapper: RNA modification mapping software for analysis of mass spectrometry data”, N Yu, C Wetzel, X Cao, PA Limbach, Analytical Chemistry (2017) doi: 10.1021/acs.analchem.7b01780 PMID: 28942636

June 2 - 6, 2024

Future ASMS conference in Anaheim, CA

Upcoming Events

Dr. Limbach

Dr. Patrick A. Limbach

Vice President for Research

Professor of Chemistry

Ohio Eminent Scholar

University of Cincinnati

Cincinnati, OH 45221-0172

Ph: 513-558-0026


Postdoctoral Research Associate, University of Utah, 1993-1994

Ph.D. The Ohio State University, 1992

B.S. Centre College, 1988


Abbreviated Intro Bio is available here

Pat Limbach is the Vice President for Research, an Ohio Eminent Scholar and Professor of Chemistry at the University of Cincinnati. He is a bioanalytical chemist with research interests in mass spectrometry, modified ribonucleic acids (RNAs), ribonucleoprotein (RNP) complexes and protein translation. After earning an undergraduate degree from Centre College in 1988, he studied under the direction of Dr. Alan G. Marshall at The Ohio State University. While there, his graduate research focused on instrumentation improvements to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. He received his PhD from OSU in 1992. He then took a postdoctoral position at the University of Utah working with Dr. James A. McCloskey. While in Utah, he worked in the areas of RNA chemistry and nucleic acid mass spectrometry.

In 1995, he joined the faculty at Louisiana State University as an Assistant Professor and was promoted to Associate Professor in 1999. In 2001, he moved to his current position in Cincinnati, being promoted to Professor in 2005. He has served as Department Head in Chemistry at UC from 2005-2010 and as Interim Associate Dean for Research and Graduate Studies in the McMicken College of Arts and Sciences in 2013. He has served as the VP for Research since 2016.

Dr. Limbach and his group seek to advance the area of mass spectrometry within modified RNAs and RNPs and collaborate extensively with researchers inside the state of Ohio and throughout the world. Dr. Limbach is a Fellow of the American Association for the Advancement of Science (AAS), an active member of the American Chemical Society, American Society for Mass Spectrometry, Sigma Xi and a lifetime member of Phi Kappa Phi.

Selected Awards and Honors:

2013 Fellow, American Association for the Advancement of Science (AAAS)

2009 Cincinnati Chemist of the Year Award, Cincinnati Section American Chemical Society

2008 Sigma Xi Young Investigator Research Award, University of Cincinnati

2006 Fellow of the Graduate School, University of Cincinnati

2003 Ohio Eminent Scholar in Mass Spectrometry

1999 Nontenured Faculty Award in the Physical Sciences, Phi Kappa Phi, LSU

1999 Award of Excellence in Undergraduate Teaching, College of Basic Sciences, LSU

1998 American Society for Mass Spectrometry Research Award Sponsored by Finnigan

1998 LSU Peter A. Soderbergh Award for Innovation in Teaching

1992 Ohio State University Albert J. Henne Graduate Research Award

1992 FACSS Tomas Hirschfeld Scholar Award

1992 Society for Applied Spectroscopy Ohio Valley Section Graduate Student Award

Research Areas:

  •  mass spectrometry

  •  modified nucleosides

  •  transfer RNAs

  •  structural investigation of nucleic acids

  •  characterization of ribonucleoprotein complexes

  •  assays for therapeutic oligonucleotides

  •  nucleic acid biomarkers in humans