Limbach Group Biological Mass Spectrometry

A Member of the Rieveschl Laboratories for Mass Spectrometry

in the College of Arts & Sciences' Department of Chemistry

RAMM Software Update (v2) now available

RoboOligo Software now available

Learn more about the National Academies Consensus study on Sequencing the Epitranscriptome

Selected Recent Publications

Robert L. Ross, Ningxi Yu, Ruoxia Zhao, Andrew Wood and Patrick A. Limbach. “Automated Identification of Modified Nucleosides during HRAM-LC-MS/MS using a Metabolomics ID Workflow with Neutral Loss Detection” Journal of the American Society for Mass Spectrometry (2023) doi:10.1021/jasms.3c00298.

Manasses Jora, Daniel Corcoran, Gwenn G. Parungao, Peter A. Lobue, Luiz F. L. Oliveira, George Stan, Balasubrahmanyam Addepalli, and Patrick A. Limbach “Higher-Energy Collisional Dissociation Mass Spectral Networks for the Rapid, Semi-automated Characterization of Known and Unknown Ribonucleoside Modifications” Analytical Chemistry (2022) 94(40):13958-13967. doi: 10.1021/acs.analchem.2c03172. PMID: 36174068.

"Chemical Amination/Imination of Carbonothiolated Nucleosides During RNA Hydrolysis” Jora M, Borland K, Abernathy S, Zhao R, Kelley M, Kellner S, Addepalli B, Limbach PA. Angew Chem Int Ed Engl. (2021) 60 3961-3966. DOI: 10.1002/anie.202010793. PMID: 33125801

“Oligonucleotide Analysis by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry in the Absence of Ion-Pair Reagents” P.A. Lobue, M. Jora, B. Addepalli, P.A. Limbach,  Journal of Chromatography A (2019) DOI: 10.1016/j.chroma.2019.02.016. PMID: 30772056.    

“Transfer RNA Modification Profiles and Codon Decoding Strategies in Methanocaldococcus jannaschii” N. Yu, M. Jora, B. Solivio, P. Thakur, C.G. Acevedo-Rocha, L. Randau, V. de Crécy-Lagard, B. Addepalli, P.A. Limbach,  Journal of Bacteriology (2019) 201 e00690-18. DOI: 10.1128/JB.00690-18 PMID: 30745370.

“RNAModMapper: RNA modification mapping software for analysis of mass spectrometry data”, N Yu, C Wetzel, X Cao, PA Limbach, Analytical Chemistry (2017) doi: 10.1021/acs.analchem.7b01780 PMID: 28942636

June 2 - 6, 2024

Future ASMS conference in Anaheim, CA

Upcoming Events

Contact Us

Contact Professor Limbach

Our group is always interested in self-motivated students and researchers who are excited about new scientific challenges and discoveries. If you are such a student, we would love to have you consider

joining our program. Interested undergraduate and graduate students should contact Professor Limbach directly.  No prior experience in our areas of research is required.

At the current time, only postdoctoral scholars with external support will be considered for positions in the group. If you have such funding and are interested, please send along a cover letter including a description of the funding (source, duration, amount, requirements), CV and the contact info for at least three references.


We are located in the 429 Rieveschl Suite on the University of Cincinnati Uptown Campus (West). Directions to the UC Campus are available here. You can obtain a pdf map of the Uptown West Campus here to locate Rieveschl Hall.

If traveling by car, you can access our labs by parking in the Clifton Court Garage (321 Clifton Court, Cincinnati OH), taking the garage elevators to the 4th floor.  Turn right upon exiting the elevators, take your first hallway to the right, and we are halfway down the hallway on the right (white doors). You will need to enter that main door to find the offices and laboratories of Professor Limbach and his group.

Professor Limbach’s office is 429K Rieveschl.  Student offices are 429C and 429S Rieveschl. Ellen Tabeling's office, along with the postdoctoral researchers in the group, is 429F Rieveschl.  All of our instrumentation, culturing and bench space are located in laboratories within the 429 Suite.

Individuals interested in the R. Marshall Wilson Mass Spectrometry Facility should go to 412 Rieveschl.

The Department of Chemistry Main Office is located in 404 Crosley Tower, which is adjacent to Rieveschl Hall.

Contact Numbers

Professor Limbach VP Research Office Ph: 513-558-0026

Rieveschl 429 Suite Labs/Offices Ph: 513-556-0920

R. Marshall Wilson Mass Spectrometry Facility Ph: 513-556-1575

Department of Chemistry Main Office Ph: 513-556-9200

Department of Chemistry Main Office Fax: 513-556-9239

Mailing Address

Our mailing address is:

301 Clifton Court

Department of Chemistry

PO Box 210172

University of Cincinnati

Cincinnati, OH 45221-0172