Limbach Group Biological Mass Spectrometry

A Member of the Rieveschl Laboratories for Mass Spectrometry

in the College of Arts & Sciences' Department of Chemistry

RAMM Software Update (v2) now available

RoboOligo Software now available

Learn more about the National Academies Consensus study on Sequencing the Epitranscriptome

Selected Recent Publications

Robert L. Ross, Ningxi Yu, Ruoxia Zhao, Andrew Wood and Patrick A. Limbach. “Automated Identification of Modified Nucleosides during HRAM-LC-MS/MS using a Metabolomics ID Workflow with Neutral Loss Detection” Journal of the American Society for Mass Spectrometry (2023) doi:10.1021/jasms.3c00298.

Manasses Jora, Daniel Corcoran, Gwenn G. Parungao, Peter A. Lobue, Luiz F. L. Oliveira, George Stan, Balasubrahmanyam Addepalli, and Patrick A. Limbach “Higher-Energy Collisional Dissociation Mass Spectral Networks for the Rapid, Semi-automated Characterization of Known and Unknown Ribonucleoside Modifications” Analytical Chemistry (2022) 94(40):13958-13967. doi: 10.1021/acs.analchem.2c03172. PMID: 36174068.

"Chemical Amination/Imination of Carbonothiolated Nucleosides During RNA Hydrolysis” Jora M, Borland K, Abernathy S, Zhao R, Kelley M, Kellner S, Addepalli B, Limbach PA. Angew Chem Int Ed Engl. (2021) 60 3961-3966. DOI: 10.1002/anie.202010793. PMID: 33125801

“Oligonucleotide Analysis by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry in the Absence of Ion-Pair Reagents” P.A. Lobue, M. Jora, B. Addepalli, P.A. Limbach,  Journal of Chromatography A (2019) DOI: 10.1016/j.chroma.2019.02.016. PMID: 30772056.    

“Transfer RNA Modification Profiles and Codon Decoding Strategies in Methanocaldococcus jannaschii” N. Yu, M. Jora, B. Solivio, P. Thakur, C.G. Acevedo-Rocha, L. Randau, V. de Crécy-Lagard, B. Addepalli, P.A. Limbach,  Journal of Bacteriology (2019) 201 e00690-18. DOI: 10.1128/JB.00690-18 PMID: 30745370.

“RNAModMapper: RNA modification mapping software for analysis of mass spectrometry data”, N Yu, C Wetzel, X Cao, PA Limbach, Analytical Chemistry (2017) doi: 10.1021/acs.analchem.7b01780 PMID: 28942636

June 2 - 6, 2024

Future ASMS conference in Anaheim, CA

Upcoming Events

Publications/Software/Databases/Book Chapters


RNA Mod Mapper - Ningxi Yu’s  software for automated annotation of RNA mass spectrometry data.

RoboOligo - Paul Sample’s  software for de novo and manual annotation of RNA mass spectrometry data.  

MO Predict - Software developed in the Limbach Group to expand the capabilities found on-line in Mongo Oligo.

Books and Contributed Chapters in Edited Books

     •  Analytical Characterization of Oligonucleotides by Mass Spectrometry Chapter 4 in Analysis of Oligonucleotides and Their Related Substances edited by D. Elder, G. Okafo, and M. Webb (2013)

     •  Matrix-Assisted Laser Desorption/Ionization in VOLUME V. Bioanalytical Approaches: MS, NMR, Isotope Methods, Spectroscopy, Ligand-Binding, and Other Analytical Chemistry Methods of the Encyclopedia of Drug Metabolism and Interactions edited by David C. Muddiman and Lucinda Cohen (2012)

     •  Analysis by Hydrophilic Interaction Chromatography - Chapter 16 in Handbook of Analysis of Oligonucleotides and Related Products edited by J. Bonilla and S. Srivatsa (2010)

     •  Mass Spectrometry of Nucleosides and Nucleic Acids - edited by Joseph A Banoub and Patrick A Limbach (2010)

     •  A Comparison of MALDI Matrices - Chapter 7 in Electrospray and MALDI Mass Spectrometry: Fundamentals, Instrumentation, Practicalities, and Biological Applications, 2nd Edition edited by Richard B. Cole (2010)

     •  Bio-MEMS Devices for Proteomics - in Bio-MEMS: Technologies and Applications edited by Wanjun Wang and Stephen A. Soper (2006)

     •  Mass Spectrometry of Nucleic Acids - Chapter 7 in Genomic Technologies: Present and Future edited by David J. Galas and Stephen J. McCormack (2002)

     •  Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing - edited by Patrick A. Limbach, John C. Owicki, Ramesh Raghavachari and Weihong Tan (2000)

Jan 6, 2022

Peer-Reviewed Publications

The majority of our peer-reviewed research publications can be accessed by searching PubMed.

     •  Search for Limbach Group Publications via PubMed

     •  Search for Free-text Limbach Group Publications via PubMedCentral

Many research publications can also be accessed via GoogleScholar.

     •  Search for Limbach Group Publications via Google Scholar

The complete listing of Limbach Group Publications can be accessed below.

     •  2021 - present publications

     •  2018 - 2020 publications

     •  2015 - 2017 publications

     •  2012 - 2014 publications

     •  2009 - 2011 publications

     •  2006 - 2008 publications

     •  2000 - 2005 publications

     •  pre-2000 publications