A Member of the Rieveschl Laboratories for Mass Spectrometry

in the College of Arts & Sciences' Department of Chemistry

Manasses Jora, Daniel Corcoran, Gwenn G. Parungao, Peter A. Lobue, Luiz F. L. Oliveira, George Stan, Balasubrahmanyam Addepalli, and Patrick A. Limbach “Higher-Energy Collisional Dissociation Mass Spectral Networks for the Rapid, Semi-automated Characterization of Known and Unknown Ribonucleoside Modifications” Analytical Chemistry (2022) Oct 11;94(40):13958-13967. doi: 10.1021/acs.analchem.2c03172. PMID: 36174068. [Link]

Priti Thakur, Jowad Atway, Patrick A Limbach and Balasubrahmanyam Addepalli, “RNA cleavage properties of nucleobase-specific RNase MC1 and cusativin are determined by the dinucleotide binding interactions in the enzyme active site” International Journal of Molecular Sciences (2022) DOI: 10.3390/ijms23137021. [Link]

Kelley, Melissa; Paulines, Mellie June; Yoshida, George; Myers, Ryan; Jora, Manasses; Levoy, Joel P.; Addepalli, Balasubrahmanyam; Benoit, Joshua B.; Limbach, Patrick A. “Ionizing radiation and chemical oxidant exposure impacts on Cryptococcus neoformans transfer RNAs” PLOS ONE (2022) 17(3)  e0266239. DOI: 10.1371/journal.pone.0266239. PMID: 35349591. [Link]

Kelley, Melissa; Uhran, Melissa; Herbert, Cassandra; Yoshida, George; Watts, Emmarie R.; Limbach, Patrick A.; Benoit, Joshua B. “Abundances of transfer RNA modifications and transcriptional levels of tRNA-modifying enzymes are sex-associated in mosquitoes” Insect Biochemistry and Molecular Biology (2022) 143 103741. DOI: 10.1016/j.ibmb.2022.103741. PMID: 35181477. [Link]

D'Ascenzo, Luigi; Popova, Anna M.; Abernathy, Scott; Sheng, Kai; Limbach, Patrick A.; Williamson, James R. “Pytheas: a software package for the automated analysis of RNA sequences and modifications via tandem mass spectrometry” Nature Communications (2022) 13(1) 2424. DOI: 10.1038/s41467-022-30057-5. PMID: 35505047. [Link]

Michel Fasnacht, Stefano Gallo, Puneet Sharma, Maximilian Himmelstoss, Patrick A. Limbach, Jessica Willi and Norbert Polacek “Dynamic 23S rRNA modification ho5C2501 benefits Escherichia coli under oxidative stress” Nucleic Acids Research (2021) doi:10.1093/nar/gkab1224. [Link]

Sameer Dixit, Alan C. Kessler, Jeremy Henderson, Xioabei Pan, Ruoxia Zhao, Gabriel Silveira D’Almeida, Sneha Kulkarni, Mary Anne T. Rubio, Eva Hegedűsová, Robert Ross, Patrick A. Limbach, Brian Green, Zdeněk Paris and Juan D. Alfonzo “Dynamic queuosine changes in tRNA couple nutrient levels to codon choice in Trypanosoma brucei” Nucleic Acids Research (2021) 49 12986-12999. doi: 10.1093/nar/gkab1204. PMID: 34883512 [Link]

Sneha Kulkarni, Mary Anne T. Rubio, Eva Hegedűsová, Robert Ross, Patrick A. Limbach, Juan D Alfonzo and Zdeněk Paris “Preferential import of Queosine-modified tRNAs into T. brucei mitochondria is critical for organellar protein synthesis” Nucleic Acids Research (2021) 49(14):8247-8260. doi: 10.1093/nar/gkab567. PMID: 34244755. PMCID: PMC8373054. [Link]

Mariana Estevez, Satenik Valesyan, Manasses Jora, Patrick A. Limbach and Balasubrahmanyam Addepalli, “Oxidative Damage to RNA is Altered by the Presence of Interacting Proteins or Modified Nucleosides” Frontiers in Molecular Biosciences (2021) 8 697149. DOI: 10.3389/fmolb.2021.697149. [Link]

Jana Jeschke, Evelyne Collignon, Clémence Al Wardi, Mohammad Krayem, Martin Bizet, Yan Jia, Emilie Calonne, Bouchra Hasabi, Zena Wimana, Renato Morandini, Rachel Deplus, Pascale Putmans, Gaurav Dube, Nitesh Singh, Alexander Koch4 Kateryna Shostak, Lara Rizzotto, Robert Ross, Soizic Garaud, Christine Desmedt, Yaccine Bareche, Francoise Rothe, Gerben Menschaert, Luis Teixeira, Mingzhou Guo, Patrick A. Limbach, Pierre Close, Alain Chariot, Eleonora Leucci6, Ghanem Ghanem, Bi-Feng Yuan, Christos Sotiriou, Jean-Christophe Marine, and François Fuks “Loss of the m6A RNA demethylase FTO promotes EMT-mediated tumor progression and confers sensitivity to Wnt inhibitors” Nature Cancer (2021) 2 611–628. DOI: 10.1038/s43018-021-00223-7. [Link]

Yasemin Yoluç, Gregor Ammann, Pierre Barraud, Manasses Jora, Patrick A. Limbach, Yuri Motorin, Virginie Marchand, Carine Tisné, Kayla Borland, Stefanie Kellner “Instrumental analysis of RNA modifications” Critical Reviews in Biochemistry and Molecular Biology (2021) 56(2) 178-204. DOI: 10.1080/10409238.2021.1887807. PMID: 33618598. [Link]

Publications 2021-present